User talk:ElNando888/Blog/Reverse Transcriptase: Difference between revisions
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<p><span class="mw-headline">[[User:Omei|Omei]] ([[User talk:Omei|talk]]) 16:59, 14 November 2013 (UTC)</span></p> | <p><span class="mw-headline">[[User:Omei|Omei]] ([[User talk:Omei|talk]]) 16:59, 14 November 2013 (UTC)</span></p> | ||
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<p><span class="mw-headline">This would seem logical, but actually, I don't think it would. It only an intuition, but if Reverse Transcriptase is like other polymerases, then it has a 3D geometry, with an entrance, a channel where the single-stranded nucleotides slide one by one, and only then, it reaches an internal "pocket" called the active site. The corollary is that the stacks have to be broken some time before the RT adds the complementary DNA base. What length exactly? no clue, but there's a hint that says that it could be as much as 6 bases. And the blocking point may not be at the base of the stack as we see it in the MFE structure. So it's hard to tell...</span></p> | <p><span class="mw-headline">This would seem logical, but actually, I don't think it would. It's only an intuition, but if Reverse Transcriptase is anything like the other polymerases, then it has a 3D geometry, with an entrance, a channel where the single-stranded nucleotides slide one by one, and only then, it reaches an internal "pocket" called the active site. The corollary is that the stacks have to be broken open some time before the RT adds the complementary DNA base. What length exactly? no clue, but there's a hint that says that it could be as much as 6 bases. And the blocking point may not be at the base of the stack as we see it in the MFE structure. So it's hard to tell...</span></p> | ||
<p>-- [[User:ElNando888|ElNando888]] ([[User talk:ElNando888|talk]]) 19:23, 14 November 2013 (UTC)</p> | <p>-- [[User:ElNando888|ElNando888]] ([[User talk:ElNando888|talk]]) 19:23, 14 November 2013 (UTC)</p> | ||
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<p><span class="mw-headline"><br /></span></p> | <p><span class="mw-headline"><br /></span></p> | ||
Revision as of 19:24, 14 November 2013
Re Unbreakable stacks
Once it gets past the bar code, wouldn't the effect of the reverse transcription being blocked be seen in the SHAPE data as a highly reactive base immediately preceding the blocked base? I don't see that in the Try 1 Bracing data.
Omei (talk) 16:59, 14 November 2013 (UTC)
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This would seem logical, but actually, I don't think it would. It's only an intuition, but if Reverse Transcriptase is anything like the other polymerases, then it has a 3D geometry, with an entrance, a channel where the single-stranded nucleotides slide one by one, and only then, it reaches an internal "pocket" called the active site. The corollary is that the stacks have to be broken open some time before the RT adds the complementary DNA base. What length exactly? no clue, but there's a hint that says that it could be as much as 6 bases. And the blocking point may not be at the base of the stack as we see it in the MFE structure. So it's hard to tell...
-- ElNando888 (talk) 19:23, 14 November 2013 (UTC)
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