User:Dennis9600

<img title="Smile" src="/wiki/extensions/TinyMCE_MW/jscripts/tiny_mce/plugins/emotions/img/smiley-smile.gif" border="0" alt="Smile" /> I have just added Vienna 2.1.1 dot plots to my publications.  I wanted to incorporate them into the report files, but it was much more convenient to just make a separate file for each submitted design.  Giving each one a unique file name that was acceptable to Python, the Windows operating system, Ghostscript, and Google Drive required a little name mangling. 

 

<img title="Innocent" src="/wiki/extensions/TinyMCE_MW/jscripts/tiny_mce/plugins/emotions/img/smiley-innocent.gif" border="0" alt="Innocent" /> There are a huge number of files this time.  I haven't had time to look at everything I uploaded to the ReportsActiveLabs folder.  Please PM me if you spot any problems.

 

Analysis Reports and Spreadsheets produced by my Python script can be found in <a href="https://drive.google.com/folderview?id=0B-rDnoMjFSH2cDFibWt6T19nRzg&usp=sharing">ReportsActiveLabs</a>.  You can bookmark this folder in your browser.  I have decided to stick with one folder rather than change with each publication cycle. 

 

In this edition of my analysis reports and spreadsheets (new items are bolded):

  1) Every lab has it's own subfolder now

  2) Vienna 2.1.1 dot plots for each submitted design!!!

  3) If the target structure contains locked, non-Canonical pairs, the Vienna tools are called with a --nsp option that allows the pairing and assigns an energy of 0 to it.  Zero may not

be the best value to use, but it seems to be better than not doing anything.

  4) Some of the labs now have a starting sequence of 'GG' instead of 'GGAAA'.  It looks like the devs have left things open for additional "tails" to be used in future labs.  For now, my script

recognizes both of the sequences that have appeared and uses the correct one for each lab.

  5)  There is a field for the Vienna 2.1.1 melt point of each design submitted.  This new field now appears in both the text reports and the spreadsheets.

My lab tool includes a forecasting tool that looks at factors that have correlated well with past synthesis scores.  The current version of the forecasting tool looks at the following factors:

    a) Whether or not the design folded correctly in EteRNA's energy model (Vienna 1.8.5)

    b) Whether or not the design folded correctly in the Vienna 2.1.1 Energy Model

    c) The melt point of the design (as reported by the EteRNA server)

    d) The percentages of C,U, and G in the design, and how far they differ from 13, 10, and 21% respectively (the Berex Strategy).

    e) log₁₀(designer's EteRNA points).  This factor is given a very low weight.

 

Things I have looked at:

I have also looked at the temperature setting of the energy model as a possible factors for my forecasting tool.  I was suprised to find that the default setting of 37C is the best setting for my forecasting tool.

 

 

Things to do:

1. I am (still) thinking about how to add Vienna 2.1.1 melt curves  to these reports.  

2. Investigate what changes EteRNA made to Vienna 1.8.5 dot plots.

2. As of 11/10/2013, about three new round of synthesis results are available.  I need to run the training portion of my forecasting tool on the new data.

3. Investigate Vienna's "ensemble diversity measure" as another possible factor for the forecasting tool to consider.  I'm capturing the diversity measure now,

but not doing anything with it yet.

4. Look for published energy parameters for non-Canonical pairs in RNA sequences.  There's got to be something better than 0 out there.

5. Look at "upgrading" to Vienna 2.1.3 (from Vienna 2.1.1) in my toolset.

 

Please let me know if you have any other things you would like to see in my lab reports.

 

Happy folding,

---Dennis9600