Reproducibility Test Lab

The Reproducibilty Test Lab is a special lab that is (will be) synthesized in each synthesis round.  The objectives for this lab are:

• Provide a consistent measure of the reproducibilty of the cloud lab synthesis results, for long term quality control,
• Determine how much of the variation in synthesis results is captured by the error estimates reported for each synthesized base,
• Provide some guidance as to where the synthesis process might be improved, in order to increase reproducibility, and
• Give those analysing the synthesis data a better understanding of the variation to expect when comparing data from different synthesis rounds.

 

Initial Sequences

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Sequence	Description of rationale for inclusion	Original SHAPE data, or expected folding
AGUCAUCUAC UACGAAAAAC ACAUUCUAUU CGAAAACGAA UAGAAUGUGU CGUAGUAGAU GAC	Cloud Lab 1 representative. Round 1. ID=2366937. Score = 94.
GCAAGGACGA AUAAGCCAUA ACCGCAGGGA AACACUGAAC GGAGCCGAAA GAGCAAGCAA UAA	Cloud Lab 4 representative. Round 2. ID=2655773. Score = 88.
AUCGAAAGAU	Very short sequence