Melting temperature: Difference between revisions
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<p>The <strong>melting temperature</strong> (usually noted T<sub>m</sub>) of an oligonucleotide ([[DNA]] or [[RNA]]) is defined as the temperature at which 50% of the [[Base Pair|base pair]]s have been broken (or in other words, where only 50% of the original base pairs remain)</p> | <p>The <strong>melting temperature</strong> (usually noted T<sub>m</sub>) of an oligonucleotide ([[DNA]] or [[RNA]]) is defined as the temperature at which 50% of the [[Base Pair|base pair]]s have been broken (or in other words, where only 50% of the original base pairs remain).</p> |
Revision as of 18:18, 17 August 2021
The melting temperature (usually noted Tm) of an oligonucleotide (DNA or RNA) is defined as the temperature at which 50% of the base pairs have been broken (or in other words, where only 50% of the original base pairs remain).