DMS: Difference between revisions
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<p dir="ltr">DMS stands for dimethyl sulfate. Basically DMS is footprinting or fingerprinting if you like, of the RNA structure. It is a chemical check of which bases are paired as they should be and which are not. Rhiju describes it as doing following. DMS reacts only with unpaired A and C nucleotides. | <p dir="ltr">DMS stands for dimethyl sulfate. Basically DMS is footprinting or fingerprinting if you like, of the RNA structure. It is a chemical check of which bases are paired as they should be and which are not. Rhiju describes it as doing following. DMS reacts only with unpaired A and C nucleotides. Nando explains, "DMS works by binding the Watson-Crick edge". As Brourd added it 1.5 - 3 days to complete.</p> | ||
<p><br />Here is a<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2701642/"> science paper</a>, the abstract do a good job of explaining what DMS is.</p> | <p><br />Here is a<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2701642/"> science paper</a>, the abstract do a good job of explaining what DMS is.</p> | ||
Revision as of 19:37, 18 September 2013
DMS stands for dimethyl sulfate. Basically DMS is footprinting or fingerprinting if you like, of the RNA structure. It is a chemical check of which bases are paired as they should be and which are not. Rhiju describes it as doing following. DMS reacts only with unpaired A and C nucleotides. Nando explains, "DMS works by binding the Watson-Crick edge". As Brourd added it 1.5 - 3 days to complete.
Here is a<a href="http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2701642/"> science paper</a>, the abstract do a good job of explaining what DMS is.