Aptamer: Difference between revisions
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<p>Like antibodies, RNA aptamers can be extremely specific to a target and are capable of discriminating between closely related molecules. RNA aptamers and antibodies can be generated to bind a similarly diverse array of targets. Antibodies, however, are composed of protein and must be produced by the immune cells of a living organism. Novel aptamers can be selected quicky in vitro and amplified by PCR.</p> | <p>Like antibodies, RNA aptamers can be extremely specific to a target and are capable of discriminating between closely related molecules. RNA aptamers and antibodies can be generated to bind a similarly diverse array of targets. Antibodies, however, are composed of protein and must be produced by the immune cells of a living organism. Novel aptamers can be selected quicky in vitro and amplified by PCR.</p> | ||
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<td>RNA aptamers are generated by selection. The selection process begins with a large library of random sequences and removes RNAs that do not bind the target or bind nonspecifically. The selected RNAs are isolated, reverse-transcribed, amplified, and the process is repeated.</td> | <td>RNA aptamers are generated by selection. The selection process begins with a large library of random sequences and removes RNAs that do not bind the target or bind nonspecifically. The selected RNAs are isolated, reverse-transcribed, amplified, and the process is repeated.</td> | ||
<td>[[File:SelexWEB.jpg|thumb]]</td> | <td>[[File:SelexWEB.jpg|thumb|300px]]</td> | ||
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Revision as of 17:35, 19 April 2013
An aptamer is a nucleic acid that binds a target molecule.
Comparison to Antibodies
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Like antibodies, RNA aptamers can be extremely specific to a target and are capable of discriminating between closely related molecules. RNA aptamers and antibodies can be generated to bind a similarly diverse array of targets. Antibodies, however, are composed of protein and must be produced by the immune cells of a living organism. Novel aptamers can be selected quicky in vitro and amplified by PCR. |
Selection of Aptamers
<tbody> </tbody>| RNA aptamers are generated by selection. The selection process begins with a large library of random sequences and removes RNAs that do not bind the target or bind nonspecifically. The selected RNAs are isolated, reverse-transcribed, amplified, and the process is repeated. |
